FXR2 Polyklonaler Antikörper

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• KD/KO Validated

FXR2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12552-1-AP

Synonyme

RNA-binding protein FXR2, FXR2P, FMR1L2, FMR1 autosomal homolog 2

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Galerie der Validierungsdaten

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  WB analysis using 12552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12552-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12552-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12552-1-AP

  IP result of anti-FXR2 (IP:12552-1-AP, 4ug; Detection:12552-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of mouse brain using 12552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12552-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HepG2 using 12552-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• RIP: See 1 publications below

Produktinformation

12552-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA FXR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FXR2 fusion protein Ag3402
• Vollständiger Name: fragile X mental retardation, autosomal homolog 2
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC020090
• Gene symbol: FXR2
• Gene ID (NCBI): 9513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FXR2 (FMR1 autosomal homolog 2), also known as: FXR2P; Fxr2h, is belong to the Fragile X gene family. FXR2 is a mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for adult hippocampal neurogenesis. Also, FXR2 acts as a regulator of adult hippocampal neurogenesis by regulating translation and/or stability of NOG mRNA, thereby preventing NOG protein expression in the dentate gyrus(Uniprot).

Publikationen

Species	Application	Title
human	RIP	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez
human	WB	Proc Natl Acad Sci U S A; ARL6IP1 mediates small-molecule-induced alleviation of Alzheimer pathology through FXR1-dependent BACE1 translation initiation; Authors - Gui-Feng Zhou ; KD Validated