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FXR1 Polyklonaler Antikörper

FXR1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13194-1-AP

Synonyme

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, Mausherzgewebe, NIH/3T3-Zellen
Erfolgreiche IPMaus-Skelettmuskelgewebe
Erfolgreiche Detektion in IHChumanes Herzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13194-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA FXR1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FXR1 fusion protein Ag3873
Vollständiger Name fragile X mental retardation, autosomal homolog 1
Berechnetes Molekulargewicht 621 aa, 70 kDa
Beobachtetes Molekulargewicht70-80 kDa
GenBank-ZugangsnummerBC028983
Gene symbol FXR1
Gene ID (NCBI) 8087
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.

Protokolle

Produktspezifische Protokolle
WB protocol for FXR1 antibody 13194-1-APProtokoll herunterladen
IHC protocol for FXR1 antibody 13194-1-APProtokoll herunterladen
IF protocol for FXR1 antibody 13194-1-APProtokoll herunterladen
IP protocol for FXR1 antibody 13194-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
mouseIF

Neuron

Fmr1 KO and fenobam treatment differentially impact distinct synapse populations of mouse neocortex.

Authors - Gordon X Wang
mouseIF

Nat Commun

Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle

Authors - Lance T. Denes
humanWB

Cell Rep

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

Authors - Xiaoli Wang

Elife

Sub-synaptic, multiplexed analysis of proteins reveals Fragile X related protein 2 is mislocalized in Fmr1 KO synapses.

Authors - Gordon X Wang
humanRIP

Elife

The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.

Authors - Belinda Baquero-Perez

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Chao (Verified Customer) (01-30-2020)

Western blot show strong band in the basal state.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart