FOXO6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FOXO6 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC (Intra), CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 19122-1-AP

Synonyme

forkhead box protein O6, FOXO6

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 19122-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19122-1-AP

  IP Result of anti-FOXO6 (IP:19122-1-AP, 3ug; Detection:19122-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19122-1-AP (FOXO6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 19122-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, Maushirngewebe, Mauslungengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

19122-1-AP bindet in WB, IP, IHC, IF, FC (Intra), CoIP, ChIP, ELISA FOXO6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: forkhead box protein O6
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: AI361654
• Gene symbol: FOXO6
• Gene ID (NCBI): 100132074
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system. FOXO6 has a isoform of 70 kDa when modified after translation (PMID:23639108).

Publikationen

Species	Application	Title
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
human	WB, IF	Elife; Inhibition of mutant EGFR in lung cancer cells triggers SOX2-FOXO6-dependent survival pathways.; Authors - S Michael Rothenberg ; KD Validated
pig	WB,ChIP,CoIP	RNA Biol; Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m6A-GPR161 signalling axis.; Authors - Xin Zong ; KD Validated
human	WB	Oncotarget; Involvement of the FOXO6 transcriptional factor in breast carcinogenesis.; Authors - François Lallemand ; KD Validated
human	IHC	Oncotarget; Elevated FOXO6 expression correlates with progression and prognosis in gastric cancer.; Authors - Jia-Hong Wang
human	IF	Mol Cell Biol; Dormant Pluripotent Cells Emerge during Neural Differentiation of Embryonic Stem Cells in a FoxO3-dependent Manner.; Authors - Megumi Ikeda

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Young-Wook (Verified Customer) (10-31-2017)

This antibody detect easily mouse Foxo1 than mouse Foxo6. There is cross reactivity with Foxo1.

• Applications: Western Blot
• Primary Antibody Dilution: 2000
• Cell Tissue Type: 293T overexpression

FOXO6 Antibody Western Blot validation (2000 dilution) in 293T overexpression (Cat no:19122-1-AP)