PSMA/GCPII Polyklonaler Antikörper

PSMA/GCPII Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13163-1-AP

Synonyme

FOLH1, PSMA, FOLH 1, FOLH, Folate hydrolase 1

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Galerie der Validierungsdaten

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  WB analysis using 13163-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13163-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13163-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 13163-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13163-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of LNCaP using 13163-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using PSMA/GCPII antibody (13163-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, humanes Lebergewebe, Mausnierengewebe, PC-3-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Gliomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 4 publications below
• FC: See 1 publications below

Produktinformation

13163-1-AP bindet in WB, IHC, IF/ICC, ELISA PSMA/GCPII und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PSMA/GCPII fusion protein Ag3812
• Vollständiger Name: folate hydrolase (prostate-specific membrane antigen) 1
• Berechnetes Molekulargewicht: 719 aa, 81 kDa
• Beobachtetes Molekulargewicht: 100 kDa, 120 kDa
• GenBank-Zugangsnummer: BC025672
• Gene symbol: PSMA
• Gene ID (NCBI): 2346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,FC	ACS Sens; Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.; Authors - Xiaoyi Gao
	IHC	Eur J Nucl Med Mol Imaging; Prostate Specifc Membrane Antigen in Human and Mouse Tissue; Authors -
mouse	IHC	J Nucl Med; Molecular imaging of Neuroendocrine Prostate Cancer by targeting Delta-like Ligand 3.; Authors - Joshua Aaron Korsen
human	WB	J Control Release; PSMA-targeted nanoparticles for specific penetration of blood-brain tumor barrier and combined therapy of brain metastases.; Authors - Jiang Ni
rat	WB	Front Pharmacol; Increasing Aspartoacylase in the Central Amygdala: The Common Mechanism of Gastroprotective Effects of Monoamine-Based Antidepressants Against Stress.; Authors - Kaiyun Yao
human	IHC	Appl Radiat Isot; Autoradiography on deparaffinized tissue sections - A feasibility study with 68Ga-labeled PET-tracers; Authors - Eva-Maria Klebermass