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FMR1 Polyklonaler Antikörper
FMR1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13755-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | K-562-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, Maus-Thymusgewebe |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Kolongewebe, humanes Gliomgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, HeLa-Zellen |
Erfolgreiche Detektion in FC (Intra) | Jurkat-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:200-1:800 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 7 publications below |
IHC | See 2 publications below |
Produktinformation
13755-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA FMR1 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | FMR1 fusion protein Ag4697 |
Vollständiger Name | fragile X mental retardation 1 |
Berechnetes Molekulargewicht | 632 aa, 71 kDa |
Beobachtetes Molekulargewicht | 59-72 kDa |
GenBank-Zugangsnummer | BC038998 |
Gene symbol | FMR1 |
Gene ID (NCBI) | 2332 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for FMR1 antibody 13755-1-AP | Protokoll herunterladen |
IHC protocol for FMR1 antibody 13755-1-AP | Protokoll herunterladen |
IF protocol for FMR1 antibody 13755-1-AP | Protokoll herunterladen |
IP protocol for FMR1 antibody 13755-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. | ||
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Cell Rep hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility | ||
Cancer Cell Int MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome. | ||
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. | ||
Cell Death Dis FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner
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Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Parisa (Verified Customer) (09-26-2023) | We used for WB, nor high specificity as we could see different bands (they could be different isoforms of the protein). But it was sensitive and we could see the band pulled down in IP for our protein of interest.
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FH Marta (Verified Customer) (02-24-2020) | not specific enough in WB. Not tested in IPworst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL.
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