FMO5 Polyklonaler Antikörper

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• KD/KO Validated

FMO5 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13699-1-AP

Synonyme

Dimethylaniline oxidase 5, FMO 5, FMO5

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Galerie der Validierungsdaten

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  WB analysis of human liver using 13699-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13699-1-AP (FMO5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13699-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13699-1-AP (FMO5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13699-1-AP

  IP result of anti-FMO5 (IP:13699-1-AP, 4ug; Detection:13699-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human breast cancer using 13699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13699-1-AP (FMO5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13699-1-AP (FMO5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

13699-1-AP bindet in WB, IHC, IP, ELISA FMO5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FMO5 fusion protein Ag4604
• Vollständiger Name: flavin containing monooxygenase 5
• Berechnetes Molekulargewicht: 533 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC035687
• Gene symbol: FMO5
• Gene ID (NCBI): 2330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism.; Authors - Shijiao Huang
mouse	WB	Biochem Pharmacol; The phenotype of a knockout mouse identifies flavin-containing monooxygenase 5 (FMO5) as a regulator of metabolic ageing.; Authors - Sandra G Gonzalez Malagon ; KO Validated
mouse	WB	Drug Metab Dispos; The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.; Authors - Min Chen
mouse	WB	Front Pharmacol; Puerarin ameliorates non-alcoholic fatty liver disease by inhibiting lipid metabolism through FMO5; Authors - Zhaoyi Li