VEGFR-1/FLT-1 Polyklonaler Antikörper

VEGFR-1/FLT-1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

39

Kat-Nr. : 13687-1-AP

Synonyme

FLT1, Fms like tyrosine kinase 1, FLT-1, FLT 1, FLT

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 13687-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13687-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13687-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 13687-1-AP

  IP result of anti-VEGFR-1/FLT-1 (IP:13687-1-AP, 5ug; Detection:13687-1-AP 1:500) with A549 cells lysate 3300ug.

• 

  IHC staining of human renal cell carcinoma using 13687-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 13687-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13687-1-AP

  Immunofluorescent analysis of HeLa cells using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Plazenta-Gewebe, Mauslungengewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 34 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• FC: See 1 publications below

Produktinformation

13687-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA VEGFR-1/FLT-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VEGFR-1/FLT-1 fusion protein Ag4558
• Vollständiger Name: fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
• Berechnetes Molekulargewicht: 1338 aa, 151 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC039007
• Gene symbol: VEGFR-1/FLT-1
• Gene ID (NCBI): 2321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders, and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF, and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short soluble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)

Publikationen

Species	Application	Title
rat	WB	ACS Appl Mater Interfaces; Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.; Authors - Lin Huang
human	WB	Hypertension; Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia; Authors - Lunbo Tan
human,mouse	WB,IHC	Biomed Pharmacother; Lychee seed polyphenol ameliorates DR via inhibiting inflammasome/apoptosis and angiogenesis in hRECs and db/db mice; Authors - Xiao-Hong Xiang
human	WB	Stem Cells; Heparanase released from mesenchymal stem cells activates integrin beta1/HIF-2alpha/Flk-1 signaling and promotes endothelial cell migration and angiogenesis.; Authors - Xinyang Hu
human	WB	J Ethnopharmacol; Diterpenoid tanshinones inhibit gastric cancer angiogenesis through the PI3K/Akt/mTOR signaling pathway; Authors - Jie-Ru Yu
mouse	WB	Clin Sci (Lond); Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability.; Authors - Mengqiu Wu

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