FH Polyklonaler Antikörper

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• KD/KO Validated

FH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 11375-1-AP

Synonyme

HsFH, Fumarase, EC:4.2.1.2

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Galerie der Validierungsdaten

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  WB analysis using 11375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11375-1-AP

  WB result of FH antibody (11375-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.

• 

  WB analysis using 11375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11375-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11375-1-AP

  IP result of anti-FH (IP:11375-1-AP, 4ug; Detection:11375-1-AP 1:8000) with mouse liver tissue lysate 3040 ug.

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  IHC staining of human placenta using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11375-1-AP (FH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11375-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FH antibody (11375-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, L02-Zellen, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

11375-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA FH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Caenorhabditis elegans, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FH fusion protein Ag1934
• Vollständiger Name: fumarate hydratase
• Berechnetes Molekulargewicht: 510 aa, 55 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC003108
• Gene symbol: FH
• Gene ID (NCBI): 2271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
human	WB	Theranostics; EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.; Authors - Feng Shi
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
human	WB	Invest Ophthalmol Vis Sci; Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.; Authors - Coupland Sarah E SE
Caenorhabditis elegans	WB	Int J Radiat Biol; Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.; Authors - Yongyan Sun