FGFR1OP Polyklonaler Antikörper

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• KD/KO Validated

FGFR1OP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 11343-1-AP

Synonyme

FOP, FGFR1 oncogene partner, CEP43, Centrosomal protein 43

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Galerie der Validierungsdaten

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  WB analysis using 11343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11343-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 11343-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11343-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11343-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11343-1-AP (FGFR1OP antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11343-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FGFR1OP antibody (11343-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 11343-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using FGFR1OP antibody (11343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HL-60-Zellen, Mausherzgewebe, MCF-7-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 10 publications below

Produktinformation

11343-1-AP bindet in WB, IHC, IF/ICC, ELISA FGFR1OP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGFR1OP fusion protein Ag1891
• Vollständiger Name: FGFR1 oncogene partner
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC011902
• Gene symbol: FGFR1OP
• Gene ID (NCBI): 11116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The fibroblast growth factor receptor 1 oncogene partner (FGFR1OP), FOP, is a centrosomal protein that is involved in the anchoring of microtubules to subcellular structures. Three isoforms have been produced by alternative splicing. A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma and progresses to acute myeloid leukemia.

Publikationen

Species	Application	Title
zebrafish	IF	Cell; Vertebrate cells differentially interpret ciliary and extraciliary cAMP.; Authors - Melissa E Truong
human	WB,IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu ; KD Validated
mouse	IF	Dev Cell; Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.; Authors - Chang Xie
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	IF	J Cell Biol; A ciliopathy complex builds distal appendages to initiate ciliogenesis.; Authors - Dhivya Kumar
human,mouse	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall