FGFBP2 Polyklonaler Antikörper

FGFBP2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13254-1-AP

Synonyme

HBp17-related protein, Fibroblast growth factor-binding protein 2, FGFBP-2, FGF-BP2, FGF-binding protein 2

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 13254-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13254-1-AP (FGFBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 13254-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13254-1-AP (FGFBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of BxPC-3 using 13254-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 13254-1-AP (FGFBP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, BxPC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

13254-1-AP bindet in WB, IF/ICC, ELISA FGFBP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGFBP2 fusion protein Ag4036
• Vollständiger Name: fibroblast growth factor binding protein 2
• Berechnetes Molekulargewicht: 223 aa, 28 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC025720
• Gene symbol: FGFBP2
• Gene ID (NCBI): 83888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor-binding protein 2 (FGFBP2), also known as 37 kDa killer-specific secretory protein (KSP37), is a member of the fibroblast growth factor binding protein family. FGFBP2 was identified as a Th1/Tc1 specific secretory protein is expressed preferentially in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and might be involved in essential processes of CTL-mediated immunity. An increase expression of FGFBP2 may be associated with atopic asthma and mild extrinsic asthma. Recently, an observation revealed that FGFBP2 correlate strongly with histology, stage and outcome in ovarian, and it might be a potential prognostic biomarker. The observed MW of FGFBP2 (37 kDa) varies from the predicated MW of 28 kDa, which may be due to the specific space conformation caused by internal disulfide bonds.

Publikationen

Species	Application	Title
human	IF	Cell Biosci; Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos; Authors - Qianqi Zhu