FGF-2 Polyklonaler Antikörper

FGF-2 Polyklonal Antikörper für WB, IHC, ELISA, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11234-1-AP

Synonyme

Basic fibroblast growth factor, BFGF, FGF2, FGF-2, FGFB, HBGF 2

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Galerie der Validierungsdaten

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  WB analysis using 11234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11234-1-AP (FGF-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 11234-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11234-1-AP (FGF-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 11234-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human FGF-2 (11234-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of K-562 using 11234-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human FGF-2 (11234-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HepG2-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, Mauslungengewebe, Mausnierengewebe, Rattenhirngewebe, Rattenlungengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 5 publications below

Produktinformation

11234-1-AP bindet in WB, IHC, FC (Intra), ELISA FGF-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fibroblast growth factor 2 (basic)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: NM_002006
• Gene symbol: FGF2
• Gene ID (NCBI): 2247
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

Publikationen

Species	Application	Title
mouse	IHC	Nat Nanotechnol; Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.; Authors - Xuefei Lu
	WB	Biomaterials; Lung endothelial cell-targeted peptide-guided bFGF promotes the regeneration after radiation induced lung injury.; Authors - Dongwei Guan
human	WB	BMC Cancer; Multi-omics sequencing revealed endostar combined with cisplatin treated non small cell lung cancer via anti-angiogenesis; Authors - Yufei Wang
human,mouse	WB	Toxicol Appl Pharmacol; Synergistic anticancer effects of ginsenoside CK and gefitinib against gefitinib-resistant NSCLC by regulating the balance of angiogenic factors through HIF-1α/VEGF; Authors - Xiaoping Song
human	WB,IHC	Wound Repair Regen; Negative pressure wound therapy is associated with upregulation of bFGF and ERK1/2 in human diabetic foot wounds.; Authors - Shao-Ling Yang
human	WB, IHC	Exp Ther Med; Expression of signal transducer and activator of transcription 3 and its phosphorylated form is significantly upregulated in patients with papillary thyroid cancer.; Authors - L I Yan