FCHSD1 Polyklonaler Antikörper

FCHSD1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23362-1-AP

Synonyme

FCH and double SH3 domains 1, FCHSD1, NWK2

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Galerie der Validierungsdaten

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  WB analysis using 23362-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23362-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23362-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23362-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23362-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 23362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 23362-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using FCHSD1 antibody (23362-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

23362-1-AP bindet in WB, IHC, IF, ELISA FCHSD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FCHSD1 fusion protein Ag19963
• Vollständiger Name: FCH and double SH3 domains 1
• Berechnetes Molekulargewicht: 690 aa, 77 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa
• GenBank-Zugangsnummer: BC047016
• Gene symbol: FCHSD1
• Gene ID (NCBI): 89848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FCHSD1 (FCH and double SH3 domains 1), also known as NWK2, is a member of the F-BAR family, which is involved in endocytosis and vesicle trafficking. FCHSD1 is expressed in mouse cochlear sensory hair cells and plays an important role in regulating the assembly or maintenance of F-actin in hair cell stereocilia and cuticular plate (PMID: 23437151). FCHSD1 also plays a role in stress response sensing and oxidative stress integration and is involved in the pathogenesis of emphysema (PMID: 34168078). FCHSD1 has three isoforms with molecular weights of 77 kDa, 46 kDa, and 40 kDa.

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.; Authors - Takahiro Kawasaki