FcRn-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FcRn-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16190-1-AP

Synonyme

alpha chain, FCGRT, FCRN, FcRn-Specific, Neonatal Fc receptor

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Galerie der Validierungsdaten

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  WB analysis using 16190-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16190-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16190-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, humanes Lebergewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

16190-1-AP bindet in WB, IHC, IF, ELISA FcRn-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Fc fragment of IgG, receptor, transporter, alpha
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: NM_004107
• Gene symbol: FCGRT
• Gene ID (NCBI): 2217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228).

Publikationen

Species	Application	Title
human	WB	Cell; Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B.; Authors - Xin Zhao ; KO Validated
pig	WB	Clin Dev Immunol; Human antibodies can cross guinea pig placenta and bind its neonatal Fc Receptor: implications for studying immune prophylaxis and therapy during pregnancy.; Authors - Struble Evi Budo EB
canine	WB,IF	Placenta; Gestation age dependent transfer of human immunoglobulins across placenta in timed-pregnant guinea pigs.; Authors - Yanqun Xu
human	WB	Int J Mol Sci; Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes; Authors - Zhonghao Li

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