FBXW11 Monoklonaler Antikörper

FBXW11 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F5A10

Kat-Nr. : 68090-1-Ig

Synonyme

BTRC2, BTRCP2, Fbw11, FBW1B, FBXW11, FBXW1B, Homologous to Slimb protein, Hos, KIAA0696

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Galerie der Validierungsdaten

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  WB analysis using 68090-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68090-1-Ig (FBXW11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 68090-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 68090-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 68090-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 68090-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68090-1-Ig (FBXW11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68090-1-Ig bindet in WB, IHC, ELISA FBXW11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FBXW11 fusion protein Ag3761
• Vollständiger Name: F-box and WD repeat domain containing 11
• Berechnetes Molekulargewicht: 542 aa, 61 kDa
• Beobachtetes Molekulargewicht: 58-62 kDa
• GenBank-Zugangsnummer: BC026213
• Gene symbol: FBXW11
• Gene ID (NCBI): 23291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.