FBXO22 Polyklonaler Antikörper

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• KD/KO Validated

FBXO22 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Rezensionen (1)

Kat-Nr. : 13606-1-AP

Synonyme

F box only protein 22, F box protein 22, F box protein FBX22p44, FBX22, FBXO22, FISTC1

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Galerie der Validierungsdaten

• 

  WB analysis using 13606-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13606-1-AP (FBXO22 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 13606-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 13606-1-AP (FBXO22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13606-1-AP

  IP result of anti-FBXO22 (IP:13606-1-AP, 4ug; Detection:13606-1-AP 1:4000) with HeLa cells lysate 1750 ug.

• 

  IHC staining of mouse heart using 13606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13606-1-AP (FBXO22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13606-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13606-1-AP (FBXO22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 23 publications below
• IHC: See 11 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

13606-1-AP bindet in WB, IP, IF, IHC, ELISA FBXO22 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXO22 fusion protein Ag4485
• Vollständiger Name: F-box protein 22
• Berechnetes Molekulargewicht: 403 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC039024
• Gene symbol: FBXO22
• Gene ID (NCBI): 26263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. FBXO22 is a key regulator of senescence induction through ubiquitylation of p53 for degradation. FBXO22 also acts as a molecular switch for the antagonistic and agonistic actions of selective estrogen receptor modulators (SERM) and determines the sensitivity of breast cancer to SERM by ubiquitylating KDM4B complexed with unliganded or SERMs‐bound estrogen receptor (ER). (PMID: 32536008, PMID: 32249768)

Publikationen

Species	Application	Title
human	WB,IHC	Cell; Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.; Authors - Luca Lignitto ; KO Validated
human	WB	Nat Commun; Targeted inhibition of the COP9 signalosome for treatment of cancer.; Authors - Anita Schlierf
human	WB	Cell Death Differ; Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.; Authors - Ping Liu ; KO Validated
human	WB,IHC,IF	Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen ; KD Validated
human	WB	Front Oncol; Pan-Cancer Analyses Reveal Oncogenic Role and Prognostic Value of F-Box Only Protein 22.; Authors - Sen Chen
human	WB	iScience; A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.; Authors - Muzammal Hussain

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Strong single band at predicted size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart