FBXO17 Polyklonaler Antikörper

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• KD/KO Validated

FBXO17 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12844-1-AP

Synonyme

FBXO26, FBX26, FBX17, F-box only protein 26, F-box only protein 17

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Galerie der Validierungsdaten

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  WB analysis using 12844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12844-1-AP (FBXO17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12844-1-AP

  WB result of FBXO17 antibody (12844-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO17 transfected HepG2 cells.

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  WB analysis of mouse heart using 12844-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12844-1-AP (FBXO17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12844-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12844-1-AP (FBXO17 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 12844-1-AP

  Immunofluorescent analysis of HepG2 cells using 12844-1-AP (FBXO17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 12844-1-AP

  Immunofluorescent analysis of HeLa cells using 12844-1-AP (FBXO17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, HepG2-Zellen, Mausherzgewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Produktinformation

12844-1-AP bindet in WB, IHC, IF/ICC, ELISA FBXO17 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXO17 fusion protein Ag3867
• Vollständiger Name: F-box protein 17
• Berechnetes Molekulargewicht: 278 aa, 31 kDa
• Beobachtetes Molekulargewicht: 29-31 kDa
• GenBank-Zugangsnummer: BC012385
• Gene symbol: FBXO17
• Gene ID (NCBI): 115290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
mouse	WB	J Biol Chem; SCFFBXO17 E3 ligase modulates inflammation by regulating proteasomal degradation of glycogen synthase kinase-3β in lung epithelia.; Authors - Tomeka Suber
human	IHC	Carcinogenesis; Systematically characterize the clinical and biological significances of 1p19q genes in 1p/19q non-codeletion glioma.; Authors - Rui-Chao Chai
human	WB,IHC	J Gene Med; Circ_0008717 promotes renal cell carcinoma progression by upregulating FBXO17 via targeting miR-217.; Authors - Siyao Shen
human	WB	Biosci Biotechnol Biochem; F-box protein 17 promotes glioma progression by regulating glycolysis pathway.; Authors - Chao Deng ; KD Validated
human,mouse	WB,IHC	Int J Med Sci; FBXO17 Inhibits the Wnt/β-Catenin Pathway and Proliferation of Ishikawa Cells; Authors - Zi-Meng Zheng