fibrosin Polyklonaler Antikörper

fibrosin Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11356-1-AP

Synonyme

FBRS, FBS, FBS1, FBSH, fibrosin, Probable fibrosin-1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11356-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11356-1-AP (fibrosin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11356-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11356-1-AP (fibrosin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11356-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11356-1-AP (fibrosin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11356-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11356-1-AP (fibrosin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11356-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11356-1-AP (fibrosin antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11356-1-AP

  Immunofluorescent analysis of HepG2 cells, using FBRS antibody 11356-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mausnierengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11356-1-AP bindet in WB, IF, IHC, ELISA fibrosin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: fibrosin fusion protein Ag1870
• Vollständiger Name: fibrosin
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 48-52 kDa
• GenBank-Zugangsnummer: BC014212
• Gene symbol: fibrosin
• Gene ID (NCBI): 64319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrosin is a lymphokine secreted by activated lymphocytes that induces fibroblast proliferation FBRS (or FBS1) gene, encoding fibrosin (or fibrosin-1), was first cloned by Prakash et al. and was found to be able to stimulate several biological activities related to scarring as a fibrogenic lymphokine. Fibrosin is secreted by activated lymphocytes that induce fibroblast proliferation. In vitro application of fibrosin stimulates expression of alpha smooth muscle actin fibers and plays an important role in up regulating the appearance of myofibroblasts during wound healing and possibly in fibrotic diseases. Fibrosin is also implicated in alcohol-induced hepatic fibrosis in vivo.