FBP1 Polyklonaler Antikörper

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• KD/KO Validated

FBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 12842-1-AP

Synonyme

Liver FBPase, Fructose-1,6-bisphosphatase 1, FBPase 1, FBP, EC:3.1.3.11

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 12842-1-AP

  WB result of FBP1 antibody (12842-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBP1 transfected MCF-7 cells.

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  WB analysis using 12842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12842-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 12842-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12842-1-AP (FBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Lebergewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12842-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA FBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBP1 fusion protein Ag3837
• Vollständiger Name: fructose-1,6-bisphosphatase 1
• Berechnetes Molekulargewicht: 338 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC012927
• Gene symbol: FBP1
• Gene ID (NCBI): 2203
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

Publikationen

Species	Application	Title
human,mouse	WB	J Med Chem; Discovery of N-Arylsulfonyl-Indole-2-Carboxamide Derivatives as Potent, Selective, and Orally Bioavailable Fructose-1,6-Bisphosphatase Inhibitors-Design, Synthesis, In Vivo Glucose Lowering Effects, and X-ray Crystal Complex Analysis.; Authors - Jie Zhou
human	WB	Am J Cancer Res; HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.; Authors - Li-Na Yang
human	WB	J Proteome Res; Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.; Authors - Yong Zhou
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
human	WB, IHC	Life Sci; Forced overexpression of FBP1 inhibits proliferation and metastasis in cholangiocarcinoma cells via Wnt/β-catenin pathway.; Authors - Wei Zhao
pig	WB	PLoS One; Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets.; Authors - Demin Cai