FATP2 Monoklonaler Antikörper

FATP2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C10D7

Kat-Nr. : 68074-1-Ig

Synonyme

ACSVL1, FACVL1, FATP 2, FATP2, Fatty acid transport protein 2, hFACVL1, HsT17226, SLC27A2, THCA CoA ligase, VLACS, VLCS

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Galerie der Validierungsdaten

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  WB analysis using 68074-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 68074-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68074-1-Ig (SLC27A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 68074-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68074-1-Ig (FATP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 68074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 68074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68074-1-Ig (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68074-1-Ig bindet in WB, IHC, ELISA FATP2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FATP2 fusion protein Ag5390
• Vollständiger Name: solute carrier family 27 (fatty acid transporter), member 2
• Berechnetes Molekulargewicht: 567 aa, 65 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC057770
• Gene symbol: SLC27A2
• Gene ID (NCBI): 11001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.