FAM107A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FAM107A Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12176-1-AP

Synonyme

TU3A, DRR1, Down-regulated in renal cell carcinoma 1

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Galerie der Validierungsdaten

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  IHC staining of human lung cancer using 12176-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12176-1-AP (FAM107A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12176-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12176-1-AP (FAM107A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 12176-1-AP

  Immunofluorescent analysis of MCF-7 cells, using FAM107A antibody 12176-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

12176-1-AP bindet in WB, IHC, IF/ICC, ELISA FAM107A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM107A fusion protein Ag2820
• Vollständiger Name: family with sequence similarity 107, member A
• Berechnetes Molekulargewicht: 144 aa, 17 kDa
• GenBank-Zugangsnummer: BC010561
• Gene symbol: FAM107A
• Gene ID (NCBI): 11170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAM107A, also named as TU3A, encodes a 144 amino-acid protein, which contains a coiled-coil domain and a nuclear localization signal. FAM107A as a transcription factor regulates some gene transcription and signal transduction in cell. With the exception of peripheral blood cells, FAM107A is widely expressed in normal tissues, but shows significant loss of expression in renal cell carcinomas and frequent loss of expression in cervical, gastric, ovarian and non small cell lung cancers. Transfection of FAM107A mRNA into cancer cell line inhibits cell growth and proliferation, supporting the evidence that the gene functions as an important tumor suppressor.

Publikationen

Species	Application	Title
mouse	WB	Hum Mol Genet; A severe mouse model of spinal muscular atrophy develops early systemic inflammation.; Authors - Bo Wan
human	WB	Front Mol Neurosci; PMID: 29632473; Authors - Jian Zhang
mouse	WB,IF	Arch Biochem Biophys; Loss of family with sequence similarity 107, member A (FAM107A) induces browning in 3T3-L1 adipocytes.; Authors - Subramani Manigandan ; KD Validated
human	WB,IHC	Cancers (Basel); FAM107A Inactivation Associated with Promoter Methylation Affects Prostate Cancer Progression through the FAK/PI3K/AKT Pathway; Authors - Shuai Ke
human	WB,IHC	Pathol Res Pract; FAM107A as a tumor suppressor in esophageal squamous carcinoma inhibits growth and metastasis; Authors - Jiale Zhang