FAF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FAF2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 16251-1-AP

Synonyme

ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

---

Galerie der Validierungsdaten

• 

  WB analysis using 16251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16251-1-AP

  WB result of FAF2 antibody (16251-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells.

• 

  IP experiment of mouse brain using 16251-1-AP

  IP Result of anti-FAF2 (IP:16251-1-AP, 3ug; Detection:16251-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

• 

  IHC staining of human breast cancer using 16251-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16251-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16251-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen, U2OS-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

16251-1-AP bindet in WB, IP, IHC, IF, ELISA FAF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAF2 fusion protein Ag9236
• Vollständiger Name: Fas associated factor family member 2
• Berechnetes Molekulargewicht: 445 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC014001
• Gene symbol: FAF2
• Gene ID (NCBI): 23197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

Publikationen

Species	Application	Title
human	IP,IF	Science; Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.; Authors - Youngdae Gwon
		Elife; USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation.; Authors - Yanfen Liu
human	WB	Elife; Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling.; Authors - Emma J Fenech
human, bovine	WB	J Biol Chem; A Ubiquitin-like Domain Recruits an Oligomeric Chaperone to a Retrotranslocation Complex in Endoplasmic Reticulum-associated Degradation.; Authors - Xu Yue Y
human	WB	J Biol Chem; Characterization of the deubiquitinating activity of USP19 and its role in endoplasmic reticulum-associated degradation.; Authors - Jin-Gu Lee
human	WB	Mol Biol Cell; gp78 functions downstream of Hrd1 to promote degradation of misfolded proteins of the endoplasmic reticulum.; Authors - Ting Zhang

Verwandte Produkte

FAF1 Polyclonal antibody

10271-1-AP

VCP Polyclonal antibody

10736-1-AP

INSIG1 Polyclonal antibody

22115-1-AP

AMFR/GP78 Polyclonal antibody

16675-1-AP

HIF-1 alpha Polyclonal antibody

20960-1-AP