FADS1 Polyklonaler Antikörper

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• KD/KO Validated

FADS1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 10627-1-AP

Synonyme

FADSD5, EC:1.14.19.44, Delta-5 desaturase, D5D, Acyl-CoA (8-3)-desaturase

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Galerie der Validierungsdaten

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  WB analysis using 10627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10627-1-AP (FADS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10627-1-AP (FADS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 10627-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 10627-1-AP (FADS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 10627-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 10627-1-AP (FADS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HeLa-Zellen, Jurkat-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

10627-1-AP bindet in WB, IHC, IF/ICC, ELISA FADS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FADS1 fusion protein Ag0978
• Vollständiger Name: fatty acid desaturase 1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007846
• Gene symbol: FADS1
• Gene ID (NCBI): 3992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FADS1, also known as Delta-5 desaturase or D5D, is a 444 amino acid protein that belongs to the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS1 is a key enzyme in polyunsaturated fatty acid (PUFA) metabolism that catalyze the conversion of linoleic acid (LA) into arachidonic acid (AA) and that of alpha-linolenic acid (ALA) into eicosapentaenoic acid (EPA).

Publikationen

Species	Application	Title
human	WB	Cell Metab; STING orchestrates the crosstalk between polyunsaturated fatty acid metabolism and inflammatory responses.; Authors - Isabelle K Vila
human	WB	Nat Commun; The lipoprotein-associated phospholipase A2 inhibitor Darapladib sensitises cancer cells to ferroptosis by remodelling lipid metabolism; Authors - Mihee Oh
human	WB	Proc Natl Acad Sci U S A; Polyunsaturated fatty acid biosynthesis pathway determines ferroptosis sensitivity in gastric cancer.; Authors - Ji-Yoon Lee
human	IHC	Stem Cells; Increased lipogenesis is critical for self-renewal and growth of breast cancer stem cells: Impact of omega-3 fatty acids.; Authors - Haiqing Luo
mouse	WB	J Lipid Res; In vivo MRS assessment of altered fatty acyl unsaturation in liver tumor formation of a TGF alpha/c-myc transgenic mouse model.; Authors - Griffitts J J
human	IHC	J Cancer; Reduced Expression of FADS1 Predicts Worse Prognosis in Non-Small-Cell Lung Cancer.; Authors - Dong Wang