FABP6 Polyklonaler Antikörper

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• KD/KO Validated

FABP6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 13781-1-AP

Synonyme

FABP6, Fatty acid binding protein 6, Gastrotropin, GT, I 15P, I BABP, I BALB, I BAP, ILBP, ILBP3, Ileal lipid binding protein, ILLBP, Intestinal 15 kDa protein

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Galerie der Validierungsdaten

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  WB analysis of mouse small intestine using 13781-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13781-1-AP (FABP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13781-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13781-1-AP (FABP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

13781-1-AP bindet in WB, IHC, ELISA, IF FABP6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FABP6 fusion protein Ag4788
• Vollständiger Name: fatty acid binding protein 6, ileal
• Berechnetes Molekulargewicht: 177 aa, 20 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC022489
• Gene symbol: FABP6
• Gene ID (NCBI): 2172
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid binding protein 6 (FABP6, also known as the ileal bile acid binding protein IBABP) is regarded as a bile acid binding protein found in the distal portion of the small intestine and may be important in maintaining bile acid homeostasis(PMID: 25754072). FABP6 is reportedly up-regulated in colorectal cancer, it has been suggested as a link between bile acids and the risk of colorectal cancer(PMID: 17909007). And also, it was showed a potential drug target for the treatment of diabetes(PMID: 27500412). There are 2 isoforms of this protein,one of which is about 14 kDa we detected.

Publikationen

Species	Application	Title
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB	J Agric Food Chem; Novel Metabolic Regulation of Bile Acid Responses to Low Cholesterol in Whole-Grain-Diet-Fed Mice.; Authors - Jinxin Liu
human	WB,IHC	J Immunol Res; FABP6 Expression Correlates with Immune Infiltration and Immunogenicity in Colorectal Cancer Cells; Authors - Wenping Lian ; KD Validated
human	WB,IHC,IF	Aging (Albany NY); FABP6 serves as a new therapeutic target in esophageal tumor; Authors - Dengfeng Zhang
human	WB	Int Immunopharmacol; Melatonin alleviates necrotizing enterocolitis by reducing bile acid levels through the SIRT1/FXR signalling axis; Authors - Jiahao Lai