FABP3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FABP3 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (1)

Kat-Nr. : 10676-1-AP

Synonyme

Mammary-derived growth inhibitor, H-FABP, Heart-type fatty acid-binding protein, Fatty acid-binding protein, heart, Fatty acid-binding protein 3

---

Galerie der Validierungsdaten

• 

  WB analysis using 10676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10676-1-AP (FABP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10676-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10676-1-AP (FABP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10676-1-AP (FABP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10676-1-AP (FABP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10676-1-AP (FABP3 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of mouse heart using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10676-1-AP (FABP3 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human heart using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10676-1-AP (FABP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10676-1-AP (FABP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10676-1-AP (FABP3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10676-1-AP (FABP3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 10676-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10676-1-AP (FABP3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse brain using 10676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FABP3 antibody (10676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 10676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FABP3 antibody (10676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Rattenhodengewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Lungengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

10676-1-AP bindet in WB, IHC, IF-P, IP, ELISA FABP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FABP3 fusion protein Ag1069
• Vollständiger Name: fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC007021
• Gene symbol: FABP3
• Gene ID (NCBI): 2170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.

Publikationen

Species	Application	Title
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB	J Neurosci; FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase67 Promoter Region.; Authors - Yui Yamamoto
mouse	WB	Int J Mol Sci; Amelioration of Nicotine-Induced Conditioned Place Preference Behaviors in Mice by an FABP3 Inhibitor; Authors - Wenbin Jia
mouse	WB	J Cell Mol Med; PMID: 21251210; Authors - Xi Lei L
mouse	WB,IF	J Biol Chem; FABP3 promotes αSyn oligomerization associated with MPTP-induced neurotoxicity.; Authors - Norifumi Shioda ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Sampath Kumar (Verified Customer) (02-26-2024)

The FABP3 antibody worked well at 1:1000 dilution on rodent skeletal muscle tissue homogenates (6ug total protein), producing bands at 15kDa. However, homogenates from Avian pectoralis muscle homogenates (6ug total protein) produced barely visible bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Avian and rodent skeletal muscle

FABP3 Antibody Western Blot validation (1:1000 dilution) in Avian and rodent skeletal muscle (Cat no:10676-1-AP)