PAR2 Polyklonaler Antikörper

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• KD/KO Validated

PAR2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 12160-1-AP

Synonyme

F2RL1, G protein coupled receptor 11, GPR11, PAR 2, PAR2, Thrombin receptor like 1

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 12160-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12160-1-AP (PAR2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

12160-1-AP bindet in WB, IF, ELISA PAR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAR2 fusion protein Ag2801
• Vollständiger Name: coagulation factor II (thrombin) receptor-like 1
• Berechnetes Molekulargewicht: 397 aa, 44 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC018130
• Gene symbol: PAR2
• Gene ID (NCBI): 2150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proteinase-activated receptors (PARs) are G protein-coupled receptors activated through cleavage of their N-termini by mainly serine proteases. The family of PARs includes four members: PAR1, PAR2, PAR3, and PAR4. PAR2, also known as F2RL1, is expressed in epithelial cells (e.g., lung, gastrointestinal tract), endothelial cells, smooth muscle cells, fibroblasts, nerves, and some immune and inflammatory cells. PAR2 knockout mice and PAR2 agonists and antagonists have implicated PAR2 as a promising target in inflammatory conditions; respiratory, gastrointestinal, metabolic, cardiovascular, and neurological dysfunction; and cancers. (PMID:23895492)

Publikationen

Species	Application	Title
		J Med Chem; Toward drugs for protease-activated receptor 2 (PAR2).; Authors - Mei-Kwan Yau
human	WB	Cells; Modulation of Intestinal Epithelial Permeability via Protease-Activated Receptor-2-Induced Autophagy.; Authors - Yuju Kim
human	WB	Biochem J; Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway.; Authors - Chunyang Du
human	WB	Oral Dis; Linagliptin's impact on lymphatic barrier and lymphangiogenesis in oral cancer with high glucose; Authors - Hongyu Wang
human	WB	Med Oncol; Long non-coding RNA AC245100.4 contributes to prostate cancer migration via regulating PAR2 and activating p38-MAPK pathway.; Authors - Chi Liu ; KD Validated
human	WB	Heliyon; Long non-coding RNA AC245100.4 activates the PI3K/AKT pathway to promote PCa cell proliferation by elevating PAR2; Authors - Ke Zhang