Emerin Polyklonaler Antikörper

Emerin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-10351

Synonyme

EDMD, EMD, emerin, LEMD5, STA

---

Galerie der Validierungsdaten

• 

  IF Staining of HeLa using CL594-10351

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Emerin antibody (CL594-10351) at dilution of 1:100.

• 

  FC experiment of HeLa using CL594-10351

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Emerin (CL594-10351) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-10351 bindet in IF, FC (Intra) Emerin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Emerin fusion protein Ag0236
• Vollständiger Name: emerin
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC000738
• Gene symbol: EMD
• Gene ID (NCBI): 2010
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011