EXOSC1 Polyklonaler Antikörper

EXOSC1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (2)

Kat-Nr. : 12585-1-AP

Synonyme

CGI 108, CSL4, Csl4p, EXOSC1, exosome component 1, hCsl4p, p13, SKI4, Ski4p

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Galerie der Validierungsdaten

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  WB analysis of SGC-7901 using 12585-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 12585-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12585-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12585-1-AP (EXOSC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12585-1-AP

  IP result of anti-EXOSC1 (IP:12585-1-AP, 4ug; Detection:12585-1-AP 1:600) with HeLa cells lysate 3000 ug.

• 

  IHC staining of human lymphoma using 12585-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12585-1-AP (EXOSC1 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen, HeLa-Zellen, humanes Magengewebe, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12585-1-AP bindet in WB, IP, IHC, ELISA EXOSC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hefe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOSC1 fusion protein Ag3286
• Vollständiger Name: exosome component 1
• Berechnetes Molekulargewicht: 195 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC022067
• Gene symbol: EXOSC1
• Gene ID (NCBI): 51013
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.

Publikationen

Species	Application	Title
yeast	WB	Am J Med Genet A; Pontocerebellar hypoplasia associated with p.Arg183Trp homozygous variant in EXOSC1 gene: A case report; Authors - Nadirah S Damseh
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Nice antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

EXOSC1 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:12585-1-AP)

FH

Andrea (Verified Customer) (01-11-2023)

Great antibody! Were incubated overnight at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC1 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:12585-1-AP)