Exo70 Polyklonaler Antikörper

Exo70 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12014-1-AP

Synonyme

2 5 3p, EX070, Exo70, Exo70p, EXOC1, EXOC7, exocyst complex component 7, KIAA1067, YJL085W

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12014-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12014-1-AP (Exo70 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12014-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12014-1-AP (Exo70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12014-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12014-1-AP (Exo70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12014-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12014-1-AP (Exo70 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12014-1-AP

  IP result of anti-Exo70 (IP:12014-1-AP, 3ug; Detection:12014-1-AP 1:600) with mouse brain tissue lysate 2640ug.

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  IHC staining of human gliomas using 12014-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12014-1-AP (Exo70 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 4 publications below
• CoIP: See 2 publications below

Produktinformation

12014-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA Exo70 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Exo70 fusion protein Ag2628
• Vollständiger Name: exocyst complex component 7
• Berechnetes Molekulargewicht: 735 aa, 83 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC011045
• Gene symbol: Exo70
• Gene ID (NCBI): 23265
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda
mouse	WB	Elife; Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis; Authors - Kunli Zhao
rat	IF	Mol Neurobiol; The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.; Authors - Matías Lira
human	WB,IF	Placenta; Exocyst complex protein expression in the human placenta.; Authors - I M Gonzalez
mouse	WB,IF,CoIP	Biol Res; Exo70 intracellular redistribution after repeated mild traumatic brain injury.; Authors - Matías Lira
human,rat	IF,CoIP	Life Sci Alliance; An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation; Authors - Priyadarshini Ravindran