Sec8 Polyklonaler Antikörper

Sec8 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11913-1-AP

Synonyme

EXOC4, exocyst complex component 4, Exocyst complex component Sec8, KIAA1699, REC8, Sec8, SEC8L1, Sec8p

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11913-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 11913-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11913-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11913-1-AP

  IP result of anti-Sec8 (IP:11913-1-AP, 4ug; Detection:11913-1-AP 1:300) with Jurkat cells lysate 2800ug.

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  IHC staining of human colon cancer using 11913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maushodengewebe, U-251-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

11913-1-AP bindet in WB, IP, IHC, ELISA Sec8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sec8 fusion protein Ag2514
• Vollständiger Name: exocyst complex component 4
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC026174
• Gene symbol: Sec8
• Gene ID (NCBI): 60412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda
mouse	WB	Elife; Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis; Authors - Kunli Zhao
human	WB	Biol Res; REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells.; Authors - Miao Liu
mouse	WB	Commun Biol; The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium; Authors - S M Niedziółka