EXOC1 Polyklonaler Antikörper

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• KD/KO Validated

EXOC1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 11690-1-AP

Synonyme

SEC3P, SEC3L1, SEC3, Exocyst complex component Sec3, Exocyst complex component 1

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 11690-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11690-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11690-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11690-1-AP

  IP result of anti-EXOC1 (IP:11690-1-AP, 3ug; Detection:11690-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human gliomas using 11690-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11690-1-AP (EXOC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of human placenta using 11690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (11690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 11690-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using EXOC1 antibody (11690-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Produktinformation

11690-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA EXOC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOC1 fusion protein Ag2303
• Vollständiger Name: exocyst complex component 1
• Berechnetes Molekulargewicht: 894 aa, 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC020650
• Gene symbol: EXOC1
• Gene ID (NCBI): 55763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.

Publikationen

Species	Application	Title
human	WB	Nature; A phosphoinositide conversion mechanism for exit from endosomes.; Authors - Katharina Ketel ; KD Validated
human	WB,IF	Nat Nanotechnol; Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.; Authors - Tanmoy Saha ; KD Validated
mouse	WB	J Cell Biol; The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.; Authors - Juan José Sáez
mouse	IF	Elife; EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice.; Authors - Yuki Osawa
human	IF	PLoS Pathog; Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape.; Authors - Yuen-Yan Chang
human	WB,IP	Retrovirology; Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation.; Authors - Mukerji Joya J