ETS2 Polyklonaler Antikörper

ETS2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12280-1-AP

Synonyme

ETS2, ETS2IT1, Protein C ets 2

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Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 12280-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12280-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12280-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12280-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12280-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12280-1-AP (ETS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12280-1-AP

  IP result of anti-ETS2 (IP:12280-1-AP, 3ug; Detection:12280-1-AP 1:600) with HeLa cells lysate 5000ug.

• 

  IHC staining of mouse spleen using 12280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 12280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12280-1-AP (ETS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, Mausnierengewebe, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

12280-1-AP bindet in WB, IP, IHC, ELISA ETS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ETS2 fusion protein Ag2929
• Vollständiger Name: v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
• Berechnetes Molekulargewicht: 469 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC017040
• Gene symbol: ETS2
• Gene ID (NCBI): 2114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; The overexpression of Tipe2 in CRC cells suppresses survival while endogenous Tipe2 accelerates AOM/DSS induced-tumor initiation.; Authors - Yan Li
human	WB	Cell Death Dis; A distal super-enhancer activates oncogenic ETS2 via recruiting MECOM in inflammatory bowel disease and colorectal cancer; Authors - Yongheng Chen
mouse	WB	Gene; Cellular senescence-Related genes define the immune microenvironment and molecular characteristics in severe asthma patients; Authors - Qibin Lin
human	IHC	BMC Med; Single-cell and spatial transcriptomics reveal metastasis mechanism and microenvironment remodeling of lymph node in osteosarcoma; Authors - Yun Liu