ESRRB Monoklonaler Antikörper

ESRRB Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

3E10C1

Kat-Nr. : 66818-1-Ig

Synonyme

DFNB35, ERR2, ERRb, ERRbeta, ERRbeta 2, ESRL2, ESRRB, ESRRB protein, estrogen related receptor beta, NR3B2

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Galerie der Validierungsdaten

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  WB analysis using 66818-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66818-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66818-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66818-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 66818-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of Caco-2 using 66818-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, Caco-2-Zellen, HeLa-Zellen, HT-29-Zellen, JAR-Zellen, LNCaP-Zellen, Maushirngewebe, NCCIT-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

66818-1-Ig bindet in WB, IHC, IF, ELISA ESRRB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ESRRB fusion protein Ag18626
• Vollständiger Name: estrogen-related receptor beta
• Berechnetes Molekulargewicht: 508 aa, 56 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC131517
• Gene symbol: ESRRB
• Gene ID (NCBI): 2103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

Publikationen

Species	Application	Title
human	IF	Stem Cell Res; Establishment of a CRISPR/Cas9-mediated GATAD2B homozygous knockout human embryonic stem cell line; Authors - Bo Wang