XPG Polyklonaler Antikörper

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• KD/KO Validated

XPG Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 11331-1-AP

Synonyme

ERCC5, ERCM2, EC:3.1.-.-, DNA repair protein complementing XP-G cells, DNA excision repair protein ERCC-5

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Galerie der Validierungsdaten

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  WB analysis using 11331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11331-1-AP (XPG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11331-1-AP

  IP result of anti-XPG (IP:11331-1-AP, 4ug; Detection:11331-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 11331-1-AP

  Immunofluorescent analysis of HeLa cells using 11331-1-AP (XPG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Daudi-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 1 publications below

Produktinformation

11331-1-AP bindet in WB, IF/ICC, IP, ELISA XPG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XPG fusion protein Ag1874
• Vollständiger Name: excision repair cross-complementing rodent repair deficiency, complementation group 5
• Berechnetes Molekulargewicht: 1186 aa, 133 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC031522
• Gene symbol: ERCC5
• Gene ID (NCBI): 2073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.

Publikationen

Species	Application	Title
human	WB	Cell; Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.; Authors - Takaaki Yasuhara ; KD Validated
human		Cancer Cell; Systematic Functional Annotation of Somatic Mutations in Cancer.; Authors - Patrick Kwok-Shing Ng
		Mol Carcinog; Associations between Expression Levels of Nucleotide Excision Repair Proteins in Lymphoblastoid Cells and Risk of Squamous Cell Carcinoma of the Head and Neck.; Authors - Peng Han
human	WB	Oncol Rep; Characterization of the nucleotide excision repair pathway and evaluation of compounds for overcoming the cisplatin resistance of non‑small cell lung cancer cell lines.; Authors - Toshihiro Suzuki ; KD Validated
human	WB	J Cancer; PMID: 31772670; Authors - Li-Ming Tan
human	WB	Biochim Biophys Acta; Stimulation of ribosomal RNA gene promoter by transcription factor Sp1 involves active DNA demethylation by Gadd45-NER pathway.; Authors - Pallavi Rajput