ERCC2 Polyklonaler Antikörper

ERCC2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10818-1-AP

Synonyme

BTF2 p80, COFS2, CXPD, EM9, ERCC2, TFIIH 80 kDa subunit, TFIIH p80, TTD, XPD, XPDC



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HEK-293-Zellen, K-562-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Lymphomgewebe, humanes Zervixkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10818-1-AP bindet in WB, IP, IF, IHC, ELISA ERCC2 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ERCC2 fusion protein Ag1038
Vollständiger Name excision repair cross-complementing rodent repair deficiency, complementation group 2
Berechnetes Molekulargewicht 87 kDa
Beobachtetes Molekulargewicht 80 kDa
GenBank-ZugangsnummerBC008346
Gene symbol ERCC2
Gene ID (NCBI) 2068
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.

Protokolle

Produktspezifische Protokolle
WB protocol for ERCC2 antibody 10818-1-APProtokoll herunterladen
IHC protocol for ERCC2 antibody 10818-1-APProtokoll herunterladen
IP protocol for ERCC2 antibody 10818-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Am J Cancer Res

Predictive value of ERCC1, ERCC2, ERCC4, and glutathione S-Transferase Pi expression for the efficacy and safety of FOLFIRINOX in patients with unresectable pancreatic cancer.

Authors - Shun Tezuka
humanIHC

Methods

Synchronous detection of miRNAs, their targets and downstream proteins in transferred FFPE sections: applications in clinical and basic research.

Authors - Zhao Jin-yao JY
humanIHC

Cancer Sci

Very low prevalence of XPD K751Q polymorphism and its association with XPD expression and outcomes of FOLFOX-4 treatment in Asian patients with colorectal carcinoma.

Authors - Lai Jiun-I JI
humanWB

Oncotarget

PMID: 27449101

Authors - Henan Zhao
humanIHC

Biomarkers

Aberrant methylation of nucleotide excision repair genes is associated with chronic arsenic poisoning

Authors - Aihua Zhang
humanIHC

J Transl Med

Proteomic analysis predicts anti-angiogenic resistance in recurred glioblastoma

Authors - Hanwool Jeon

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Zizhao (Verified Customer) (08-15-2018)

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1: 1000
  • Cell Tissue Type: human keratinocytes