EPSTI1 Polyklonaler Antikörper

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• KD/KO Validated

EPSTI1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11627-1-AP

Synonyme

Epithelial-stromal interaction protein 1

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Galerie der Validierungsdaten

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  WB analysis of mouse spleen using 11627-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11627-1-AP (EPSTI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 11627-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11627-1-AP (EPSTI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11627-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11627-1-AP (EPSTI1 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

11627-1-AP bindet in WB, IHC, IF, ELISA EPSTI1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPSTI1 fusion protein Ag2157
• Vollständiger Name: epithelial stromal interaction 1 (breast)
• Berechnetes Molekulargewicht: 410 aa, 47 kDa
• Beobachtetes Molekulargewicht: 38-47 kDa
• GenBank-Zugangsnummer: BC023660
• Gene symbol: EPSTI1
• Gene ID (NCBI): 94240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Real-time PCR revealed the highest expression in the placenta; strong expression in the small intestine, spleen, salivary gland, and testes; and low expression levels in normal breast and several other tissues. The expression of EPSTI1 was upregulated (range 5.6-72.1) in all 14 breast carcinomas tested. Microdissection revealed that both tumor and stromal tissue overexpressed EPSTI1. (PubMed: 11991720)

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Acta Pharmacol Sin; EPSTI1 promotes monocyte adhesion to endothelial cells in vitro via upregulating VCAM-1 and ICAM-1 expression.; Authors - Yan-Rou Bei ; KD Validated
human	WB	Elife; KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition.; Authors - Chaitra Rao
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB	Mol Cancer; Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study.; Authors - Erfei Chen
human	IHC	Oxid Med Cell Longev; Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer; Authors - Mujing Ke