EPS15 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

EPS15 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241485B7

Kat-Nr. : 84179-1-PBS

Synonyme

241485B7, AF 1P, AF1P, MLLT5, Protein AF 1p

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  WB analysis using 84179-1-RR (same clone as 84179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84179-1-RR (EPS15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84179-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 84179-1-RR (same clone as 84179-1-PBS)

  WB result of EPS15 antibody (84179-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS15 transfected MCF-7 cells. This data was developed using the same antibody clone with 84179-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01095-1

  Cytometric bead array standard curve of MP01095-1, EPS15 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84179-1-PBS. Detection antibody: 84179-4-PBS. Standard: Ag35282. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84179-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84179-1-PBS against Human EPS15 was performed. The affinity constant is 3.07 nM.

• 

  Affinity and Kinetic Characterization of 84179-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84179-1-RR against Human EPS15 were performed. The affinity constant is 2.72 nM.

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Geprüfte Anwendungen

Produktinformation

84179-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA EPS15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EPS15 fusion protein Ag35282
• Vollständiger Name: epidermal growth factor receptor pathway substrate 15
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_001981.3
• Gene symbol: EPS15
• Gene ID (NCBI): 2060
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPS15 was originally identified as a substrate for the kinase activity of the epidermal growth factor receptor (EGFR). EPS15 has a tripartite structure comprising an NH2-terminal portion, which contains three EH domains, a central putative coiled-coil region, and a COOH-terminal domain containing multiple copies of the amino acid triplet Aspartate-Proline-Phenylalanine (PMID: 10481267). EPS15 is phosphorylated on tyrosine residues following EGFR activation (PMID: 7689153).