EPHX2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EPHX2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 10833-1-AP

Synonyme

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

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Galerie der Validierungsdaten

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  WB analysis of A549 using 10833-1-AP

  WB result of EPHX2 antibody (10833-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells.

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  WB analysis of HEK-293 using 10833-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10833-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 10833-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 10833-1-AP

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse large intestine using 10833-1-AP

  IP result of anti-EPHX2 (IP:10833-1-AP, 4ug; Detection:10833-1-AP 1:300) with mouse large intestine tissue lysate 3200 ug.

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  IP experiment of HEK-293 using 10833-1-AP

  IP result of anti-EPHX2 (IP:10833-1-AP, 4ug; Detection:10833-1-AP 1:500) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human colon cancer using 10833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10833-1-AP (EPHX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10833-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10833-1-AP (EPHX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10833-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10833-1-AP (EPHX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HEK-293T-Zellen, Maus-Kolongewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche IP: Maus-Dickdarmgewebe, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

10833-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA EPHX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPHX2 fusion protein Ag1283
• Vollständiger Name: epoxide hydrolase 2, cytoplasmic
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC013874
• Gene symbol: EPHX2
• Gene ID (NCBI): 2053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

Publikationen

Species	Application	Title
mouse	WB,IF	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
human	WB	Int J Biol Sci; Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury; Authors - Juan Zhang
rat	WB	Biochim Biophys Acta Mol Basis Dis; Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ; Authors - Yang Zhou
human	WB,IHC	Front Oncol; EPHX2 Inhibits Colon Cancer Progression by Promoting Fatty Acid Degradation.; Authors - Yiran Zhou
chicken	WB	Arch Biochem Biophys; Mitochondrial P450-dependent arachidonic acid metabolism by TCDD-induced hepatic CYP1A5; conversion of EETs to DHETs by mitochondrial soluble epoxide hydrolase.; Authors - Labitzke Erin M EM
rat	WB	Biochem Biophys Res Commun; Adeno-associated virus-mediated in vivo suppression of expression of EPHX2 gene modulates the activity of paraventricular nucleus neurons in spontaneously hypertensive rats.; Authors - Xiaoming Zhu