CD39/ENTPD1 Polyklonaler Antikörper

CD39/ENTPD1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14211-1-AP

Synonyme

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse placenta using 14211-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (ENTPD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14211-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14211-1-AP

  human placenta lysates were subjected to SDS PAGE followed by western blot with 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14211-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14211-1-AP (CD39/ENTPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14211-1-AP

  Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14211-1-AP

  Immunofluorescent analysis of HeLa cells using 14211-1-AP (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 14211-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug ENTPD1 antibody (14211-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Plazenta-Gewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Lebergewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

14211-1-AP bindet in WB, IHC, IF, FC, ELISA CD39/ENTPD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD39/ENTPD1 fusion protein Ag5429
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 1
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 50 kDa，70-100 kDa
• GenBank-Zugangsnummer: BC047664
• Gene symbol: ENTPD1
• Gene ID (NCBI): 953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa and 34 kDa) produced by alternative splicing.

Publikationen

Species	Application	Title
human,mouse	WB,IF	Int J Biol Sci; CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence; Authors - Zhenni Liu
human	IF	Genomics Proteomics Bioinformatics; Single-cell Immune Landscape of Human Recurrent Miscarriage.; Authors - Feiyang Wang
human	IHC	Oncotarget; Expression of CD38 in myeloma bone niche: A rational basis for the use of anti-CD38 immunotherapy to inhibit osteoclast formation.; Authors - Federica Costa
rat	IHC	Am J Physiol Gastrointest Liver Physiol; Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.; Authors - Moyuru Hayashi
human	WB	BMC Med Genomics; Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.; Authors - Xing-Cheng Zhao
rat	WB	J Gerontol A Biol Sci Med Sci; Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract Remodeling; Authors - Lori A Birder