NSE/ENO2-specific Polyklonaler Antikörper

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• KD/KO Validated

NSE/ENO2-specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 55235-1-AP

Synonyme

Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE, NSE/ENO2-specific

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 55235-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 55235-1-AP

  WB result of NSE/ENO2-specific antibody (55235-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE/ENO2-specific transfected U-251 cells.

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  WB analysis of HepG2 using 55235-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55235-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55235-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 55235-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293-Zellen, HepG2-Zellen, Maushirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

55235-1-AP bindet in WB, IHC,ELISA NSE/ENO2-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: enolase 2 (gamma, neuronal)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: NM_001975
• Gene symbol: ENO2
• Gene ID (NCBI): 2026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. This antibody is specific to ENO2 and has no cross reaction to ENO1 and ENO3.

Publikationen

Species	Application	Title
mouse	WB	Mol Med Rep; miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase.; Authors - Chunli Xu