NSE Polyklonaler Antikörper

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• KD/KO Validated

NSE Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Kat-Nr. : 10149-1-AP

Synonyme

ENO2, Neural enolase, Gamma enolase, Enolase 2, EC:4.2.1.11

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Galerie der Validierungsdaten

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  WB analysis using 10149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10149-1-AP (NSE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10149-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10149-1-AP (NSE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 10149-1-AP

  WB result of NSE antibody (10149-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells.

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  WB analysis of mouse brain using 10149-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10149-1-AP (NSE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10149-1-AP

  IP result of anti-NSE (IP:10149-1-AP, 4ug; Detection:10149-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10149-1-AP (NSE antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10149-1-AP (NSE antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10149-1-AP (NSE antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10149-1-AP (NSE antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10149-1-AP (NSE antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 10149-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10149-1-AP (NSE antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 10149-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NSE antibody (10149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 11 publications below

Produktinformation

10149-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NSE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NSE fusion protein Ag0196
• Vollständiger Name: enolase 2 (gamma, neuronal)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC002745
• Gene symbol: NSE
• Gene ID (NCBI): 2026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.

Publikationen

Species	Application	Title
mouse	IF	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	WB	Mater Today Bio; In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation.; Authors - Dan Zhang
mouse	WB	Mater Today Bio; Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.; Authors - Luyu Wang
human	WB	Aging (Albany NY); Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation.; Authors - Fang Li
goat	IF	Cell Cycle; Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.; Authors - Zhimin Wang
human	WB	Cell Biosci; Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer; Authors - Qiong Wang