EMG1 Polyklonaler Antikörper

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• KD/KO Validated

EMG1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11965-1-AP

Synonyme

NEP1, Grcc2f, EC:2.1.1.-, C2F, 18S rRNA Psi1248 methyltransferase

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11965-1-AP

  WB result of EMG1 antibody (11965-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMG1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 11965-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:3000 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 11965-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11965-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11965-1-AP (EMG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11965-1-AP

  WB result of EMG1 antibody (11965-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMG1 transfected HeLa cells.

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  IP experiment of HeLa using 11965-1-AP

  IP result of anti-EMG1 (IP:11965-1-AP, 4ug; Detection:11965-1-AP 1:400) with HeLa cells lysate 1080ug.

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  IF Staining of HeLa using 11965-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EMG1 antibody (11965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11965-1-AP bindet in WB, IF/ICC, IP, ELISA EMG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EMG1 fusion protein Ag2575
• Vollständiger Name: EMG1 nucleolar protein homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 244 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC055314
• Gene symbol: EMG1
• Gene ID (NCBI): 10436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EMG1, also named as NEP1, is a highly conserved protein initially identified as "Essential for Mitotic Growth" in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood.

Publikationen

Species	Application	Title
		Mol Cell Proteomics; Nucleolar enrichment of brain proteins with critical roles in human neurodevelopment.; Authors - Lukasz Slomnicki
mouse	IF	Biochim Biophys Acta; Growth arrest in the ribosomopathy, Bowen-Conradi syndrome, is due to dramatically reduced cell proliferation and a defect in mitotic progression.; Authors - Joy Armistead
goat	WB	Int J Mol Sci; Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods; Authors - Zhipeng Sun