ELF1 Polyklonaler Antikörper

ELF1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55029-1-AP

Synonyme

E74 like factor 1, ELF1

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Galerie der Validierungsdaten

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  WB analysis of A431 using 55029-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55029-1-AP (ELF1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 55029-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55029-1-AP (ELF1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 55029-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 55029-1-AP (ELF1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 55029-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55029-1-AP (ELF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55029-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55029-1-AP (ELF1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55029-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55029-1-AP (ELF1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Jurkat-Zellen, K-562-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55029-1-AP bindet in WB, ELISA ELF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: E74-like factor 1 (ets domain transcription factor)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 70-95 kDa
• GenBank-Zugangsnummer: NM_172373
• Gene symbol: ELF1
• Gene ID (NCBI): 1997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELF1, also named as ETS-related transcription factor Elf-1, is originally cloned from a human T-cell cDNA library by hybridization with a probe encoding the DNA binding domain (ETS domain) of the human Ets-1 cDNA. Based on its preferential expression in embryonic lymphoid organs (thymus and spleen), a wide variety of epithelial cells and fetal liver as well as in adult haematopoietic tissues, including thymus, spleen and bone marrow, Elf-1 emerged as a potential key regulator of haematopoietic gene expression. Consistent with this notion, Elf-1 has been shown to be a direct upstream regulator of genes important for haematopoiesis such as Scl, Fli-1, Lyl-1, Runx1 and Lmo2 . Elf-1 has also been shown to be important for blood vessel development, a process that is closely linked to early haematopoiesis during embryonic development. Elf-1 has been reported to take part in the transcriptional control of major regulators of blood vessel development such as Tie1, Tie2, angiopoietin-2, the vascular endothelial growth factor receptor 1 (VEGFR1), the endothelial nitric-oxide synthase (eNOS) and endoglin . Functional activity of Ets proteins is modulated at multiple levels. It is known that ELF-1 appears in the cytoplasm as a 80 KDa protein that is O -glycosylated and phosphorylated in order to be translocated into the nucleus where it can be detected as a 98 KDa protein. After dephosphorylation, the protein is degraded through the proteasome pathway. The inactive form of Elf-1 is an 80-kDa protein that lacks DNA-binding activity and is confined to the cytoplasm of the cell. Phosphorylation and O-linked glycosylation increase the molecular weight of Elf-1 to 98 kDa, the active form; 98 kDa Elf-1 binds to the promoter of the gene that codes for CD3ζ inducing its transcription.