ELF1 Polyklonaler Antikörper

ELF1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, EMSA, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 22565-1-AP

Synonyme

E74 like factor 1, ELF1

---

Galerie der Validierungsdaten

• 

  WB analysis using 22565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 22565-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22565-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22565-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 22565-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22565-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 22565-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22565-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22565-1-AP (ELF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22565-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22565-1-AP (ELF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 22565-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 22565-1-AP (ELF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, A431-Zellen, HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

22565-1-AP bindet in WB, IHC, IF, EMSA, chIP, ELISA ELF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELF1 fusion protein Ag14689
• Vollständiger Name: E74-like factor 1 (ets domain transcription factor)
• Berechnetes Molekulargewicht: 619 aa, 67 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC030507
• Gene symbol: ELF1
• Gene ID (NCBI): 1997
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELF1, also named as ETS-related transcription factor Elf-1, is originally cloned from a human T-cell cDNA library by hybridization with a probe encoding the DNA binding domain (ETS domain) of the human Ets-1 cDNA. Based on its preferential expression in embryonic lymphoid organs (thymus and spleen), a wide variety of epithelial cells and fetal liver as well as in adult haematopoietic tissues, including thymus, spleen and bone marrow, Elf-1 emerged as a potential key regulator of haematopoietic gene expression. Consistent with this notion, Elf-1 has been shown to be a direct upstream regulator of genes important for haematopoiesis such as Scl, Fli-1, Lyl-1, Runx1 and Lmo2 . Elf-1 has also been shown to be important for blood vessel development, a process that is closely linked to early haematopoiesis during embryonic development. Elf-1 has been reported to take part in the transcriptional control of major regulators of blood vessel development such as Tie1, Tie2, angiopoietin-2, the vascular endothelial growth factor receptor 1 (VEGFR1), the endothelial nitric-oxide synthase (eNOS) and endoglin . Functional activity of Ets proteins is modulated at multiple levels. It is known that ELF-1 appears in the cytoplasm as a 80 KDa protein that is O -glycosylated and phosphorylated in order to be translocated into the nucleus where it can be detected as a 98 KDa protein. After dephosphorylation, the protein is degraded through the proteasome pathway. The inactive form of Elf-1 is an 80-kDa protein that lacks DNA-binding activity and is confined to the cytoplasm of the cell. Phosphorylation and O-linked glycosylation increase the molecular weight of Elf-1 to 98 kDa, the active form; 98 kDa Elf-1 binds to the promoter of the gene that codes for CD3ζ inducing its transcription.

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	EMSA	J Autoimmun; rs9459874 and rs1012656 in CCR6/FGFR1OP confer susceptibility to primary biliary cholangitis.; Authors - Yuki Hitomi
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
human	chIP	J Exp Clin Cancer Res; TNFAIP8 promotes AML chemoresistance by activating ERK signaling pathway through interaction with Rac1.; Authors - Yihua Pang
human	IHC	BMC Cancer; Novel biomarkers to predict treatment response and prognosis in locally advanced rectal cancer undergoing neoadjuvant chemoradiotherapy; Authors - Bingjie Guan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Iru (Verified Customer) (06-18-2020)

There are other non-specific multiple bands as well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells