ELAVL4 Monoklonaler Antikörper

ELAVL4 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B1F5

Kat-Nr. : CL488-67835

Synonyme

ELAV like protein 4, ELAVL4, Hu antigen D, HUD, PNEM

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Galerie der Validierungsdaten

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  IF Staining of mouse brain using CL488-67835

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 ELAVL4 antibody (CL488-67835, Clone: 1B1F5 ) at dilution of 1:200.

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  IF Staining of U2OS using CL488-67835

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 ELAVL4 antibody (CL488-67835, Clone: 1B1F5 ) at dilution of 1:200.

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  FC experiment of U2OS using CL488-67835

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ELAVL4 (CL488-67835, Clone:1B1F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maushirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in FC: U2OS-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67835 bindet in IF, FC (Intra) ELAVL4 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ELAVL4 fusion protein Ag3678
• Vollständiger Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Berechnetes Molekulargewicht: 380 aa, 42 kDa
• Beobachtetes Molekulargewicht: 32-40 kDa
• GenBank-Zugangsnummer: BC036071
• Gene symbol: ELAVL4
• Gene ID (NCBI): 1996
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C