ELAVL2 Polyklonaler Antikörper

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• KD/KO Validated

ELAVL2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IP, RIP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (1)

Kat-Nr. : 14008-1-AP

Synonyme

HUB, Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2

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Galerie der Validierungsdaten

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  WB analysis of mouse cerebellum using 14008-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14008-1-AP (ELAVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 14008-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14008-1-AP (ELAVL2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse cerebellum using 14008-1-AP

  IP result of anti-ELAVL2 (IP:14008-1-AP, 4ug; Detection:14008-1-AP 1:30000) with mouse cerebellum tissue lysate 3000 ug.

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  IHC staining of human brain using 14008-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14008-1-AP (ELAVL2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14008-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14008-1-AP (ELAVL2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe
• Erfolgreiche IP: Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• RIP: See 3 publications below

Produktinformation

14008-1-AP bindet in WB, IHC, IP, RIP, ELISA, IF ELAVL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELAVL2 fusion protein Ag5105
• Vollständiger Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC030692
• Gene symbol: ELAVL2
• Gene ID (NCBI): 1993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].

Publikationen

Species	Application	Title
mouse	IP	Sci Adv; Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development; Authors - Yuki Takada
human	WB,IF,RIP	Nucleic Acids Res; HuB and HuD repress telomerase activity by dissociating HuR from TERC.; Authors - Xiaolei Cheng ; KD Validated
mouse	RIP	EMBO Rep; ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles.; Authors - Yuzuru Kato
chicken	IF	Cell Rep; miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability.; Authors - Ronald J Parchem
human	WB	Hum Mol Genet; ELAVL2-regulated transcriptional and splicing networks in human neurons link neurodevelopment and autism.; Authors - Stefano Berto ; KD Validated
		iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jacopo (Verified Customer) (09-03-2019)

Very noce and specific bands in western blot. Also works for RIP

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioma stem cells