EIF5B Polyklonaler Antikörper

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• KD/KO Validated

EIF5B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 13527-1-AP

Synonyme

Translation initiation factor IF-2, KIAA0741, IF2, eIF-5B, eIF 5B

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13527-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13527-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 13527-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 13527-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 13527-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below

Produktinformation

13527-1-AP bindet in WB, IHC, IF/ICC, ELISA EIF5B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF5B fusion protein Ag4404
• Vollständiger Name: eukaryotic translation initiation factor 5B
• Berechnetes Molekulargewicht: 1220 aa, 139 kDa
• Beobachtetes Molekulargewicht: 175 kDa
• GenBank-Zugangsnummer: BC032639
• Gene symbol: EIF5B
• Gene ID (NCBI): 9669
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit. A single crossreactive polypeptide of 175 kDa was detected, whereas the predicted size of the protein was 139 kDa. This size discrepancy may be the result of posttranslational modifications of EIF5B or, perhaps more likely, of unusual behavior in SDS-PAGE caused by the highly charged N-terminal region of EIF5B (PMID: 10200264 ).

Publikationen

Species	Application	Title
human	WB	Cell Mol Life Sci; eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B.; Authors - Eunah Kim
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang ; KD Validated
human	WB	bioRxiv; Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation; Authors - Yuanhui Mao ; KD Validated
human	WB	bioRxiv; LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins; Authors - James A Saba ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bill (Verified Customer) (12-23-2019)

This antibody give every strong signal on EIF5B.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colerectal cancer cells