EIF5 Polyklonaler Antikörper

EIF5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 11155-1-AP

Synonyme

eIF-5, EIF 5A, eIF 5

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Galerie der Validierungsdaten

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  WB analysis using 11155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11155-1-AP (EIF5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11155-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11155-1-AP (EIF5 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human pancreas cancer using 11155-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11155-1-AP (EIF5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11155-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF5 antibody (11155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11155-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF5 antibody (11155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11155-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA EIF5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF5 fusion protein Ag1629
• Vollständiger Name: eukaryotic translation initiation factor 5
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC032866
• Gene symbol: EIF5
• Gene ID (NCBI): 1983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor 5, also named as EIF5, is a 431 amino acid protein, which contains one W2 domain and belongs to the eIF-2-beta/eIF-5 family. EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. EIF5 plays multiple roles in translation initiation.

Publikationen

Species	Application	Title
goat	WB,IF	Int J Mol Sci; The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats; Authors - Zhen Gao
human	WB	Nucleic Acids Res; An interaction between eIF4A3 and eIF3g drives the internal initiation of translation; Authors - Jeeyoon Chang
mouse,human	WB	bioRxiv; Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology; Authors - Jimmy Ly
human	IHC	Front Oncol; CircRNA-100338 Is Associated With mTOR Signaling Pathway and Poor Prognosis in Hepatocellular Carcinoma.; Authors - Xiu-Yan Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Adrianna (Verified Customer) (10-24-2022)

Very good antibody for WB application in cell line lysate. Works perfectly at 1:500 dilution in 5% BSA in TBST either at 4 deg C over night or for 2h at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MCF7