EIF4H Polyklonaler Antikörper

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• KD/KO Validated

EIF4H Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11012-1-AP

Synonyme

eIF 4H, EIF4H, KIAA0038, WBSCR1, WSCR1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11012-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11012-1-AP (EIF4H antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11012-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11012-1-AP (EIF4H antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11012-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11012-1-AP (EIF4H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

11012-1-AP bindet in WB, ELISA EIF4H und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF4H fusion protein Ag1468
• Vollständiger Name: eukaryotic translation initiation factor 4H
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC010021
• Gene symbol: EIF4H
• Gene ID (NCBI): 7458
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

eukaryotic initiation factor (eIF)4H, which encoded by the gene for HUMORFU_1, functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. And its homolog in human, WBSCR1, located on 7q11, is commonly missed in patients suffered the Williams-Beuren syndrome, which is a complex neurodevelopmental disorder characterized by cardiovascular defects, cerebral dysplasias and a peculiar cognitive-behavioral profile. EIF4H has two kind of isoforms. The short isoform(~25kDa) is the predominant isoform and is expressed alone in liver and skeletal muscle. This is a rabbit anti-EIF4H polyclonal antibody raised against part of N-terminal chain of human EIF4H, and can recognize two kinds of isoform.

Publikationen

Species	Application	Title
human	WB	J Virol; Small interfering RNAs that deplete the cellular translation factor eIF4H impede mRNA degradation by the virion host shutoff protein of herpes simplex virus.; Authors - Sarma Nandini N ; KD Validated