EIF4G1 Monoklonaler Antikörper

EIF4G1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10G8

Kat-Nr. : CL488-67199

Synonyme

DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-67199

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 EIF4G1 antibody (CL488-67199, Clone: 2B10G8 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67199 bindet in IF EIF4G1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF4G1 fusion protein Ag8357
• Vollständiger Name: eukaryotic translation initiation factor 4 gamma, 1
• Berechnetes Molekulargewicht: 1600 aa, 176 kDa
• Beobachtetes Molekulargewicht: 240-250 kDa
• GenBank-Zugangsnummer: BC007788
• Gene symbol: EIF4G1
• Gene ID (NCBI): 1981
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )