EIF4E2 Polyklonaler Antikörper

EIF4E2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, RIP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12227-1-AP

Synonyme

4E LP, 4EHP, eIF 4E type 2, eIF4E like protein 4E LP, eIF4E type 2, EIF4E2, EIF4EL3, IF4e, mRNA cap binding protein 4EHP

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 12227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12227-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12227-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12227-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 12227-1-AP

  IP result of anti-EIF4E2 (IP:12227-1-AP, 4ug; Detection:12227-1-AP 1:500) with MCF-7 cells lysate 1280ug.

• 

  IHC staining of human breast cancer using 12227-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 12227-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 12227-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12227-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausnierengewebe, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

12227-1-AP bindet in WB, IP, RIP, IHC, ELISA EIF4E2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF4E2 fusion protein Ag2864
• Vollständiger Name: eukaryotic translation initiation factor 4E family member 2
• Berechnetes Molekulargewicht: 245 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC021690
• Gene symbol: EIF4E2
• Gene ID (NCBI): 9470
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human	WB,RIP	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
human	WB	iScience; The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.; Authors - Limei Zou
human	WB,IHC	Eur J Med Res; N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma; Authors - Aierpati Maimaiti
human	WB	Nat Struct Mol Biol; Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer; Authors - Yoichiro Sugimoto